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https://olympias.lib.uoi.gr/jspui/handle/123456789/23261Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Gagro, A. | en |
| dc.contributor.author | McCloskey, N. | en |
| dc.contributor.author | Challa, A. | en |
| dc.contributor.author | Holder, M. | en |
| dc.contributor.author | Grafton, G. | en |
| dc.contributor.author | Pound, J. D. | en |
| dc.contributor.author | Gordon, J. | en |
| dc.date.accessioned | 2015-11-24T19:31:25Z | - |
| dc.date.available | 2015-11-24T19:31:25Z | - |
| dc.identifier.issn | 0019-2805 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/23261 | - |
| dc.rights | Default Licence | - |
| dc.subject | Adult | en |
| dc.subject | Animals | en |
| dc.subject | Antigens, CD40/*immunology | en |
| dc.subject | Antigens, CD5/*analysis | en |
| dc.subject | B-Lymphocyte Subsets/*immunology | en |
| dc.subject | Cell Culture Techniques | en |
| dc.subject | Cell Cycle/immunology | en |
| dc.subject | Cell Division/immunology | en |
| dc.subject | DNA/biosynthesis | en |
| dc.subject | Fetal Blood/immunology | en |
| dc.subject | Humans | en |
| dc.subject | Immunophenotyping | en |
| dc.subject | Infant, Newborn | en |
| dc.subject | Lymphocyte Activation/immunology | en |
| dc.subject | Mice | en |
| dc.subject | Palatine Tonsil/immunology | en |
| dc.subject | Receptors, Antigen, B-Cell/*immunology | en |
| dc.subject | Signal Transduction/*immunology | en |
| dc.title | CD5-positive and CD5-negative human B cells converge to an indistinguishable population on signalling through B-cell receptors and CD40 | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/11012773 | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 2000 | - |
| heal.abstract | Whether CD5 on B cells marks a subset functionally distinct from the conventional CD5 negative (CD5neg) adult population or is more an indicator of activation, remains contentious. Here we have investigated whether CD5 positive (CD5pos) and CD5neg B cells can be distinguished in terms of their response to surrogate signals aimed to model, in vitro, T-cell dependent (TD) and T-independent (TI) encounters with antigen in vivo: the predominantly CD5pos B-cell population found in cord blood, CD5 B cells positively selected from tonsils and their CD5neg counterparts, were compared. Neonatal B cells displayed a near-identical phenotype to that of adult CD5pos B cells, being characterized by uniform immunoglobulin M (IgM), immunoglobulin D (IgD), CD23 and CD44 coexpression. When cultured with anti-IgM maintained at high density on CD32-tranfected mouse L cells to model TI responses or on CD40 ligand (CD40L)-bearing L cells (with or without captured anti-IgM) to model TD encounters, DNA synthesis was stimulated to a similar extent in all three populations. Focusing on CD5 and CD23, we found that - although the signals delivered promoted distinct profiles of expression - under each condition of activation, the phenotypes that emerged for adult CD5pos and CD5neg B cells were remarkably similar. Neonatal B cells displayed a greater diminution in CD5 expression than adult CD5pos B cells following CD40 signals but otherwise the two populations again behaved similarly. The inclusion of interleukin-4 (IL-4) to cultures where cells were costimulated via surface (s)IgM and CD40 resulted in a complete loss of CD5 expression and a corresponding hyperexpression of CD23, irrespective of the population studied. The near-identical response of CD5pos and CD5neg B cells to surrogate TD or TI signals in vitro and their convergence to indistinguishable phenotypes is wholly supportive of CD5 being a fluctuating marker of activation rather than it delineating functionally distinct subsets. | en |
| heal.journalName | Immunology | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
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