Comparative immunostaining of T-associated plasma cells and other lymph-node cells in paraffin sections (Journal article)

Papadimitriou, C. S./ Stephanaki-Nikou, S. N./ Malamou-Mitsi, V. D.


The immunoperoxidase technique after trypsinization was used on paraffin sections of 24 lymph nodes with reactive lymphadenitis and abundant nests of T-associated plasma cells. These cells were negative for all the markers investigated, which were: intracytoplasmic immunoglobulins (CIg), a1-antichymotrypsin(a1-ACT), a1-antitrypsin(a1-AT), lysozyme(Lz) and fibronectin. Other categories of cells were positive or negative depending on their type. The best markers for polymorphs proved to be a1-AT and Lz, and for monocytes and histiocytes a1-ACT and Lz. Sinus histiocytes in particular were much more constantly and strongly positive for a1-ACT than for Lz. Endothelial cells appeared almost always positive for a1-ACT and were also occasionally positive for a1-AT. Fibronectin was consistently positive in mast cells and sometimes positive in other cells, especially those of monohistiocytic origin. Our present findings are against a B-cell or monohistiocytic origin for T-associated plasma cells.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Chymotrypsin/analysis/antagonists & inhibitors,Fibronectins/analysis,Histiocytes/chemistry,Histocytochemistry,Humans,Immunoenzyme Techniques,Immunoglobulins/analysis,Lymph Nodes/*pathology,Lymphadenitis/*pathology,Mast Cells/chemistry,Monocytes/chemistry,Muramidase/analysis,Plasma Cells/chemistry,alpha 1-Antichymotrypsin,alpha 1-Antitrypsin/analysis
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/22929
ISSN: 0340-6075
Item type: journalArticle
Link: http://www.ncbi.nlm.nih.gov/pubmed/6136117
Item language: en
Item access scheme: campus
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Publication date: 1983
Abstract: The immunoperoxidase technique after trypsinization was used on paraffin sections of 24 lymph nodes with reactive lymphadenitis and abundant nests of T-associated plasma cells. These cells were negative for all the markers investigated, which were: intracytoplasmic immunoglobulins (CIg), a1-antichymotrypsin(a1-ACT), a1-antitrypsin(a1-AT), lysozyme(Lz) and fibronectin. Other categories of cells were positive or negative depending on their type. The best markers for polymorphs proved to be a1-AT and Lz, and for monocytes and histiocytes a1-ACT and Lz. Sinus histiocytes in particular were much more constantly and strongly positive for a1-ACT than for Lz. Endothelial cells appeared almost always positive for a1-ACT and were also occasionally positive for a1-AT. Fibronectin was consistently positive in mast cells and sometimes positive in other cells, especially those of monohistiocytic origin. Our present findings are against a B-cell or monohistiocytic origin for T-associated plasma cells.
Journal name: Virchows Arch B Cell Pathol Incl Mol Pathol
Journal type: peer-reviewed
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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