Distribution of prothymosin alpha in rat tissues (Journal article)

Haritos, A. A./ Tsolas, O./ Horecker, B. L.


A radioimmunoassay, using a rabbit antiserum directed against thymosin alpha 1, was employed to detect the presence of crossreacting peptides in rat tissues. Highest concentrations were present in thymus, but thymosin alpha 1 cross-reacting material was also detected in brain, liver, kidney, lung, and spleen, in amounts ranging from 15% to 65% of the quantities found in thymus. In each case, the major immunoreactive peptide, after extraction and purification by a procedure that avoids proteolytic modification, was identified as prothymosin alpha, a peptide containing approximately equal to 112 amino acid residues. Prothymosin alpha is believed to be the endogenous peptide from which thymosin alpha 1 and other fragments are formed by proteolytic modification during the preparation of thymosin fraction 5. No peptides corresponding in size and chromatographic behavior to thymosin alpha 1 were detected with the extraction procedure employed.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Amino Acids/analysis,Animals,Brain Chemistry,Kidney/analysis,Liver/analysis,Lung/analysis,Protein Precursors/*analysis,Radioimmunoassay/methods,Rats,Spleen/analysis,Thymosin/*analogs & derivatives/analysis,Thymus Gland/*analysis,Tissue Distribution
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/22411
ISSN: 0027-8424
Link: http://www.ncbi.nlm.nih.gov/pubmed/6584887
http://www.pnas.org/content/81/5/1391.full.pdf
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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