Distribution of the regulatory subunit of type II cAMP-dependent protein kinase in Y-79 retinoblastoma cells. Effect of butyrate (Journal article)

Kyritsis, A. P./ Kapoor, C. L./ Chader, G. J.

An indirect immunofluorescence technique was employed to determine the intracellular distribution of the regulatory subunit of type II cyclic adenosine 3':5'-monophosphate-dependent protein kinase (RII) in Y-79 human retinoblastoma cells growing in monolayer culture. During the initial phases of growth (6 hr-6 days after seeding), RII was confined to the cytoplasmic areas of the Y-79 cells, seemingly, the Golgi apparatus. Treatment of cells for 3-5 days with 4 mM butyrate resulted in translocation of RII from cytoplasm to nuclei (mainly nucleoli) of cells. In a later stage of growth (24-day-old cultures), RII immunofluorescence was significantly decreased in all compartments within the untreated cells. In contrast, about 70% of the butyrate-treated cells yet showed nucleoli and/or cytoplasmic localization of RII at this stage. The nucleolar appearance of RII was parallel to the growth arrest and differentiation induced by butyrate.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Biological Transport/drug effects,Butyrates/*pharmacology,Butyric Acid,Butyric Acids/*pharmacology,Carrier Proteins/*metabolism,Cell Division,Cell Line,Cell Nucleolus/metabolism,Cytoplasm/metabolism,Eye Neoplasms/*metabolism,Fluorescent Antibody Technique,Humans,*Intracellular Signaling Peptides and Proteins,Retinoblastoma/*metabolism
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/22409
ISSN: 0146-0404
Link: http://www.ncbi.nlm.nih.gov/pubmed/3528034
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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