Dynamic interplay between the neutral glycosphingolipid CD77/Gb3 and the therapeutic antibody target CD20 within the lipid bilayer of model B lymphoma cells (Journal article)

Jarvis, R. M./ Chamba, A./ Holder, M. J./ Challa, A./ Smith, D. C./ Hodgkin, M. N./ Lord, J. M./ Gordon, J.

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dc.contributor.authorJarvis, R. M.en
dc.contributor.authorChamba, A.en
dc.contributor.authorHolder, M. J.en
dc.contributor.authorChalla, A.en
dc.contributor.authorSmith, D. C.en
dc.contributor.authorHodgkin, M. N.en
dc.contributor.authorLord, J. M.en
dc.contributor.authorGordon, J.en
dc.rightsDefault Licence-
dc.subjectAntibodies/*immunology/therapeutic useen
dc.subjectAntigens, CD20/*immunology/metabolismen
dc.subjectApoptosis/drug effectsen
dc.subjectBiological Markersen
dc.subjectBurkitt Lymphoma/*immunology/*metabolism/pathologyen
dc.subjectCell Line, Tumoren
dc.subjectCholera Toxin/pharmacologyen
dc.subjectLipid Bilayersen
dc.subjectMembrane Microdomains/drug effects/metabolismen
dc.subject*Models, Biologicalen
dc.subjectProto-Oncogene Proteins c-bcl-2/genetics/metabolismen
dc.subjectShiga Toxins/pharmacologyen
dc.titleDynamic interplay between the neutral glycosphingolipid CD77/Gb3 and the therapeutic antibody target CD20 within the lipid bilayer of model B lymphoma cellsen
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.abstractThe centroblast-specific differentiation marker CD77 (Gb(3)), is the receptor for Shiga-like toxin (SLT). The dynamic relationship between Gb(3)/CD77 and key B-cell membrane proteins was studied in Burkitt's lymphoma cells with a focus on CD20. Engagement of Gb(3)/CD77 with SLT-B reduced the amount of CD20 and CXCR4 available, but levels of BCR, MHC Class II, CD21, CD27 and CD54 remained unchanged. Cholesterol depletion promoted a decrease in the number of sites accessed by CD20, CXCR4 and Gb(3)/CD77 antibodies. Constitutive localisation of Gb(3)/CD77 to lipid rafts was unperturbed by either SLT-B binding or cholesterol depletion, whereas the opposite was true for CD20. The effects were specific to SLT-B, highlighted by the inability of cholera toxin B-subunit to alter CD20 availability. Thus, the binding of Gb(3)/CD77 by its cognate ligand transmits information within the lipid bilayer of model lymphoma cells to impact the behaviour of selective proteins, most notably CD20, via a mechanism influenced by the level of cholesterol within the membrane.en
heal.journalNameBiochem Biophys Res Communen
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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