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https://olympias.lib.uoi.gr/jspui/handle/123456789/22294Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Panopoulou, E. | en |
| dc.contributor.author | Gillooly, D. J. | en |
| dc.contributor.author | Wrana, J. L. | en |
| dc.contributor.author | Zerial, M. | en |
| dc.contributor.author | Stenmark, H. | en |
| dc.contributor.author | Murphy, C. | en |
| dc.contributor.author | Fotsis, T. | en |
| dc.date.accessioned | 2015-11-24T19:23:23Z | - |
| dc.date.available | 2015-11-24T19:23:23Z | - |
| dc.identifier.issn | 0021-9258 | - |
| dc.identifier.uri | https://olympias.lib.uoi.gr/jspui/handle/123456789/22294 | - |
| dc.rights | Default Licence | - |
| dc.subject | Animals | en |
| dc.subject | Carrier Proteins/physiology | en |
| dc.subject | Cattle | en |
| dc.subject | Cells, Cultured | en |
| dc.subject | DNA-Binding Proteins/*physiology | en |
| dc.subject | Endosomes/*physiology | en |
| dc.subject | Endothelium, Vascular/*physiology | en |
| dc.subject | Kinetics | en |
| dc.subject | Phosphorylation | en |
| dc.subject | Promoter Regions, Genetic | en |
| dc.subject | Receptors, Transforming Growth Factor beta/physiology | en |
| dc.subject | Signal Transduction/*physiology | en |
| dc.subject | Smad Proteins | en |
| dc.subject | Trans-Activators/*physiology | en |
| dc.subject | Zinc Fingers/physiology | en |
| dc.subject | rab5 GTP-Binding Proteins/genetics/metabolism | en |
| dc.title | Early endosomal regulation of Smad-dependent signaling in endothelial cells | en |
| heal.type | journalArticle | - |
| heal.type.en | Journal article | en |
| heal.type.el | Άρθρο Περιοδικού | el |
| heal.identifier.primary | 10.1074/jbc.M107983200 | - |
| heal.identifier.secondary | http://www.ncbi.nlm.nih.gov/pubmed/11877415 | - |
| heal.identifier.secondary | http://www.jbc.org/content/277/20/18046.full.pdf | - |
| heal.language | en | - |
| heal.access | campus | - |
| heal.recordProvider | Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής | el |
| heal.publicationDate | 2002 | - |
| heal.abstract | Transforming growth factor beta (TGFbeta) receptors require SARA for phosphorylation of the downstream transducing Smad proteins. SARA, a FYVE finger protein, binds to membrane lipids suggesting that activated receptors may interact with downstream signaling molecules at discrete endocytic locations. In the present study, we reveal a critical role for the early endocytic compartment in regulating Smad-dependent signaling. Not only is SARA localized on early endosomes, but also its minimal FYVE finger sequence is sufficient for early endosomal targeting. Expression of a SARA mutant protein lacking the FYVE finger inhibits downstream activin A signaling in endothelial cells. Moreover, a dominant-negative mutant of Rab5, a crucial protein for early endosome dynamics, causes phosphorylation and nuclear translocation of Smads leading to constitutive (i.e. ligand independent) transcriptional activation of a Smad-dependent promoter in endothelial cells. As inhibition of endocytosis using the K44A negative mutant of dynamin and RN-tre did not lead to activation of Smad-dependent transcription, the effects of the dominant-negative Rab5 are likely to be a consequence of altered membrane trafficking of constitutively formed TGFbeta/activin type I/II receptor complexes at the level of early endosomes. The results suggest an important interconnection between early endosomal dynamics and TGFbeta/activin signal transduction pathways. | en |
| heal.journalName | J Biol Chem | en |
| heal.journalType | peer-reviewed | - |
| heal.fullTextAvailability | TRUE | - |
| Appears in Collections: | Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ | |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Panopoulou-2002-Early endosomal regu.pdf | 453.08 kB | Adobe PDF | View/Open |
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