Effects of 3-methylcholanthrene and aspirin co-administration on ALDH3A1 in HepG2 cells (Journal article)

Sotiropoulou, M./ Pappas, P./ Marselos, M.

The effects of two different protocols of 3-methylcholanthrene (3MC) and aspirin co-administration were studied in a well-established human hepatoma cell line (HepG2). During this work, we have performed toxicity tests for cell viability/cell proliferation as well as studies on the expression of ALDH3A1 after exposure of HepG2 cells to 3MC or/and aspirin. For the evaluation of toxic concentrations of 3MC and aspirin, the WST-1 test was used. WST-1 is a reliable cytotoxicity test which is based on the cleavage of the tetrazolium salt WST-1 to formazan by mitochondrial enzymes of living cells. A broad range of drug concentrations for either 3MC (0.25-50.0 microM) or aspirin (0.05-10.0 mM) were used for cell exposure, in several periods of time. The expression of ALDH3A1 in HepG2 cells showed typical time- and dose-response curves of induction after application of 3MC (1-5 days, 1.5-5.0 microM, respectively). When cells were firstly exposed to 3MC (2.5 and 5.0 microM) and then to aspirin (0.25 mM), the induced ALDH3A1 activity was further enhanced in a statistically significant way (P<0.05). On the contrary, when aspirin application was preceded 3MC exposuring a statistically significant decrease in ALDH3A1 inducibility was observed, as compared with the application of 3MC alone.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Aldehyde Dehydrogenase/*metabolism,Aspirin/*administration & dosage,Carcinoma, Hepatocellular/*drug therapy/*enzymology/pathology,Cell Survival/drug effects,Drug Interactions,Glutathione Transferase/metabolism,Humans,Liver Neoplasms/*drug therapy/*enzymology/pathology,Methylcholanthrene/*administration & dosage,Tumor Cells, Cultured
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/22132
ISSN: 0009-2797
Link: http://www.ncbi.nlm.nih.gov/pubmed/11306048
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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