Effects of cotinine on sperm motility, membrane function, and fertilizing capacity in vitro (Journal article)
Sofikitis, N./ Takenaka, M./ Kanakas, N./ Papadopoulos, H./ Yamamoto, Y./ Drakakis, P./ Miyagawa, I.
We evaluated the effect of cotinine on sperm fertilizing capacity in vitro. Human spermatozoa were washed and re-suspended in medium containing albumin and various concentrations of cotinine (0, 100, 200, 400, or 800 ng/ml). After an 8-h incubation period, sperm motility, hypoosmotic swelling test (HOST) outcome, and the percentage of hyperactivated spermatozoa were assayed. Aliquots of spermatozoa were then processed for the zona-free hamster oocyte sperm penetration assay (SPA) or hamster ooplasmic injections. Spermatozoa exposed to concentrations of cotinine equal to 400 or 800 ng/ml demonstrated significantly smaller outcomes for all of the above with the exception of after hamster ooplasmic injections, where high cotinine concentrations did not affect sperm viability or sperm capacity to undergo decondensation and activate hamster oocytes. It appears that cotinine concentrations of 400 or 800 ng/ml exert a detrimental effect on sperm motility, membrane function, and the ability to undergo capacitation. In addition, the current findings suggest that smokers with a high seminal plasma cotinine concentration who participate in assisted reproduction programs may be treated with intracytoplasmic sperm injections (ICSI) rather than conventional in vitro fertilization (IVF) trials.
|Institution and School/Department of submitter:||Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής|
|Keywords:||Animals,Cell Membrane/drug effects/physiology,Cell Survival/drug effects,Cotinine/*adverse effects,Cricetinae,Female,Fertilization in Vitro/*drug effects,Humans,Infertility, Male/chemically induced/physiopathology,Male,Microinjections,Oocytes/physiology,Smoking/*adverse effects,Sperm Motility/*drug effects,Sperm-Ovum Interactions/*drug effects|
|Appears in Collections:||Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)|
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