Effects of cyclic AMP-elevating hormones and autacoids on LPS-activated rat peritoneal, bronchoalveolar and hepatic (Kupffer) macrophages (Journal article)

Kondomerkos, D. J./ Kalamidas, S. A./ Kotoulas, O. B.


Peritoneal, bronchoalveolar and hepatic (Kupffer) macrophages activated in vitro by endotoxin, exhibit alterations in nitric oxide production when certain hormones or other biologically active agents (autacoids) are present in the culture medium. They also show changes in acid beta-glucuronidase activities and morphological changes concerning cell size and general appearance. Agents known to elevate the intracellular levels of cyclic AMP, e.g. adrenalin, prostaglandin E2 and dopamine, increase the nitric oxide production in all three types of macrophage. The addition of H-89, an inhibitor of protein kinase A, abolishes the increase in nitric oxide production. Adrenalin also increases the extracellular activity of beta-glucuronidase. The results of this work suggest that cyclic AMP-elevating hormones and autacoids affect the functions of endotoxin-activated macrophages, such as the production of nitric oxide and the activity of acid beta-glucuronidase.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Animals,Autacoids/*metabolism,Cyclic AMP/*metabolism,Cyclic AMP-Dependent Protein Kinases/metabolism,Dinoprostone/metabolism,Dopamine/metabolism/pharmacology,Epinephrine/metabolism/pharmacology,Female,Hormones/metabolism,Kupffer Cells/drug effects/metabolism,Lipopolysaccharides/*pharmacology,*Macrophage Activation/drug effects,Macrophages, Alveolar/drug effects/*metabolism,Macrophages, Peritoneal/drug effects/*metabolism/ultrastructure,Male,Nitric Oxide/metabolism,Prostaglandin-Endoperoxide Synthases/metabolism,Rats,Rats, Wistar
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/22100
ISSN: 0263-6484
Link: http://www.ncbi.nlm.nih.gov/pubmed/15655807
http://onlinelibrary.wiley.com/store/10.1002/cbf.1205/asset/1205_ftp.pdf?v=1&t=h1ym2ubx&s=95b04e818d9d7b365a7e5ccc087a553190313540
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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