Efforts to create an artificial testis: culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment (Journal article)

Sofikitis, N./ Pappas, E./ Kawatani, A./ Baltogiannis, D./ Loutradis, D./ Kanakas, N./ Giannakis, D./ Dimitriadis, F./ Tsoukanelis, K./ Georgiou, I./ Makrydimas, G./ Mio, Y./ Tarlatzis, V./ Melekos, M./ Miyagawa, I.


Induction of meiotic and post-meiotic alterations of male germ cells in vitro has been the target of several research efforts since 1960. However, to date, the establishment of an ideal culture system in which spermatogonial stem cells can be maintained and directed to proliferate and undergo meiosis and complete spermiogenesis does not exist. This is attributed to the difficulties concerning the isolation and purification of defined subpopulations of germ cells and the establishment of male germ cell lines. In addition, there is no adequate knowledge regarding the optimal biochemical conditions that promote the survival and differentiation of germ cells in long-term cultures. This review focuses on the methodologies that have been proved sufficient to achieve differentiation of cultured male germ cells. Furthermore, the factors regulating spermatogenesis and the technical prerequisites to achieve differentiation of cultured male germ cells are described. Finally, the role of in vitro cultures of immature diploid germ cells in the therapeutic management of men negative for haploid cells in their testes and the subsequent potential genetic and epigenetic risks are discussed.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Animals,Apoptosis/physiology,Cell Culture Techniques/*methods,Cell Differentiation/physiology,Coculture Techniques,Humans,Male,Meiosis/physiology,Oocytes/physiology,Seminiferous Tubules/*physiology,Sertoli Cells/physiology,Signal Transduction/physiology,Spermatogenesis/*physiology,Spermatozoa/*cytology
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/22029
ISSN: 1355-4786
Link: http://www.ncbi.nlm.nih.gov/pubmed/15817525
http://humupd.oxfordjournals.org/content/11/3/229.full.pdf
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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