Expression of apoptosis-inducing factor (AIF) in keratoacanthomas and squamous cell carcinomas of the skin (Journal article)

Skyrlas, A./ Hantschke, M./ Passa, V./ Gaitanis, G./ Malamou-Mitsi, V./ Bassukas, I. D.

The complex biological trait 'susceptibility to apoptosis' is a nosological feature distinguishing squamous cell carcinomas (SCC) from keratoacanthomas (KA). The purpose of this study was to compare the expression of apoptosis-inducing factor (AIF), a major effector of the caspase-independent apoptosis pathway, in formalin-fixed SCC (N = 23) and KA (N = 29) resection specimens. SCC express statistically significant more AIF than KA both as proportion of AIF+ cells by immunohistochemistry (median: 54% vs 33%; P < 0.01) and as total AIF protein content by western blot quantification (six-fold increased; P < 0.01). However, the contribution of AIF to apoptosis, measured as fraction of apoptotic nuclei with overt DNA fragmentation by the TUNEL method that co-express AIF translocated to nucleus, is significantly less prevalent among SCC (median: 19% vs 48% in KA; P < 0.01). These findings indicate to a distinctive involvement of AIF in the progression of certain epithelial skin tumors that might be exploited as a promising treatment target.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Apoptosis,Apoptosis Inducing Factor/*metabolism,Carcinoma, Squamous Cell/*metabolism/pathology,Cell Nucleus/metabolism/pathology,Humans,In Situ Nick-End Labeling,Keratoacanthoma/*metabolism/pathology,Microscopy, Fluorescence,Retrospective Studies,Skin Diseases/*metabolism/pathology,Skin Neoplasms/*metabolism/pathology
ISSN: 1600-0625
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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