Expression of bcl2 family proteins and active caspase 3 in classical Hodgkin's lymphomas (Journal article)

Bai, M./ Papoudou-Bai, A./ Horianopoulos, N./ Grepi, C./ Agnantis, N. J./ Kanavaros, P.


The expression of various bcl2 family proteins has been reported in Hodgkin and Reed-Sternberg cells, but the proteins bad, bid, and bim have not been analyzed in classical Hodgkin's lymphomas (HLs). This study aimed to investigate the expression of the proteins bcl2, bcl-xl, mcl1, bax, bak, bad, bid, bim, and active caspase 3, and the TUNEL (terminal deoxynucleotidyl transferase-mediated in situ labeling) index to gain further insight into the apoptosis profile of classical HLs. A high expression of the proteins bcl2, bcl-xl, mcl1, bax, bak, bad, bid, and bim in HRS cells was found in 27 of 101 (27%), 95 of 101 (94%), 27 of 97 (29%), 73 of 95 (77%), 37 of 102 (36%), 85 of 94 (90%), 19 of 109 (17%), and 43 of 91 (47%) cases, respectively. The high expression of bcl-xl, bax, and bad in HRS cells in most classical HLs indicates that these proteins may play predominant roles in the regulation of apoptosis in classical HLs. Active caspase 3-positive and TUNEL-positive Reed-Sternberg cells were detected in 47 of 70 (67%; range, 0%-12%) and 60 of 71 (85%; range, 0%-19%) cases, respectively. Significant positive correlations were found between bax/bcl2 (P = .002), bad/bcl2 (P = .020), bad/bcl-xl (P = .003), and bim/mcl1 (P = .036). Based on these findings, it could be hypothesized that the antiapoptotic proteins bcl2, bcl-xl, and mcl1 may counteract the expression of the proapoptotic proteins bax, bad, and bim, thereby contributing to the survival of Reed-Sternberg cells.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Apoptosis/physiology,Apoptosis Regulatory Proteins/analysis,BH3 Interacting Domain Death Agonist Protein/analysis,Caspase 3/*biosynthesis,Hodgkin Disease/metabolism/*pathology/physiopathology,Humans,Immunohistochemistry,In Situ Nick-End Labeling,Membrane Proteins/analysis,Neoplasm Proteins/analysis,Proto-Oncogene Proteins/analysis,Proto-Oncogene Proteins c-bcl-2/analysis/*biosynthesis,Reed-Sternberg Cells/chemistry/pathology,bcl-2 Homologous Antagonist-Killer Protein/analysis,bcl-2-Associated X Protein/analysis,bcl-Associated Death Protein/analysis,bcl-X Protein/analysis
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/21711
ISSN: 0046-8177
Link: http://www.ncbi.nlm.nih.gov/pubmed/16949642
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