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  4. Τμήμα Ιατρικής
  5. Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά) - ΙΑΤ
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Please use this identifier to cite or link to this item: https://olympias.lib.uoi.gr/jspui/handle/123456789/21639
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dc.contributor.authorTzavaras, T.en
dc.contributor.authorEftaxia, S.en
dc.contributor.authorTavoulari, S.en
dc.contributor.authorHatzi, P.en
dc.contributor.authorAngelidis, C.en
dc.date.accessioned2015-11-24T19:16:17Z-
dc.date.available2015-11-24T19:16:17Z-
dc.identifier.issn1019-6439-
dc.identifier.urihttps://olympias.lib.uoi.gr/jspui/handle/123456789/21639-
dc.rightsDefault Licence-
dc.subjectAnimalsen
dc.subjectAntineoplastic Agents, Hormonal/pharmacologyen
dc.subjectBlotting, Northernen
dc.subjectDNA/metabolismen
dc.subjectDNA Primers/chemistryen
dc.subjectDensitometryen
dc.subjectDexamethasone/pharmacologyen
dc.subjectDiethylstilbestrol/pharmacologyen
dc.subjectDose-Response Relationship, Drugen
dc.subjectEstradiol/pharmacologyen
dc.subjectGenes, Dominanten
dc.subjectMiceen
dc.subjectNIH 3T3 Cellsen
dc.subjectPlasmids/metabolismen
dc.subjectProgesterone/pharmacologyen
dc.subjectRNA/chemistryen
dc.subjectRNA-Directed DNA Polymerase/*biosynthesis/metabolismen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectTransfectionen
dc.subjectTumor Suppressor Protein p53/metabolismen
dc.titleFactors influencing the expression of endogenous reverse transcriptases and viral-like 30 elements in mouse NIH3T3 cellsen
heal.typejournalArticle-
heal.type.enJournal articleen
heal.type.elΆρθρο Περιοδικούel
heal.identifier.secondaryhttp://www.ncbi.nlm.nih.gov/pubmed/12964010-
heal.languageen-
heal.accesscampus-
heal.recordProviderΠανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικήςel
heal.publicationDate2003-
heal.abstractRetroviral reverse transcriptase (RT) plays a definite role in retroviral life cycle and is essential for the process of retrotransposition. We investigated the RNA expression of endogenous reverse transcriptases (enRTs) in the NIH3T3 mouse genome using, as a probe, a mixture of RT-PCR generated reverse transcriptase products potentially detecting a large number of RTs following treatment with different agents. We found that the expression of enRTs is induced approximately 500-fold following 5'-azacytidine-treatment. Amongst steroid hormones used such as estradiol, diethylstilbestrol, progesterone and dexamethasone only the latter was effective in inducing enRTs up to 4-fold at a concentration of 10(-7) M. Expression of a mouse dominant-negative form of p53 protein in cell clones resulted in induction of 20- to 50-fold, whereas C2-ceramide in a 4-fold induction at concentrations of 20-80 micro M. In a parallel analysis, the respective expression of the transposable viral-like 30 elements (VL30s) was also measured. Their expression was induced up to 50-fold by 5'-azacytidine, overexpression of the p53 gene and C2-ceramide at 80 micro M. It was also induced approximately 3- to 5-fold following estradiol, diethylstilbestrol or progesterone treatment and 30-fold by dexamethasone. Collectively, our results suggest that such stimuli inducing enRTs might play a role in the activation of transcription and retrotransposition of VL30.en
heal.journalNameInt J Oncolen
heal.journalTypepeer-reviewed-
heal.fullTextAvailabilityTRUE-
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