Fine needle aspiration biopsy-RT-PCR molecular analysis of thyroid nodules: a useful preoperative diagnostic tool (Journal article)

Letsas, K. P./ Andrikoula, M./ Tsatsoulis, A.


Fine needle aspiration biopsy (FNAB)-based cytology is an accurate preoperative diagnostic method for the evaluation of thyroid nodules. Despite its high specificity and sensitivity, the results are non-diagnostic in a significant number of cases, commonly due to inadequacy of the samples or the presence of undetermined cellular morphology. Overlap-ping cytologic features among follicular-derived lesions are the most common causes of cyto-histopathologic discordances. The management of thyroid nodules with a non-diagnostic FNAB remains controversial. The significant probability of malignancy imposes either the repeat of the procedure or direct surgery. Recent studies have demonstrated the important role of molecular-based techniques such as RT-PCR in the diagnosis of thyroid lesions. Follicular cells obtained from the remaining material within the needle can be used for RNA extraction and then for RT-PCR amplification of specific mRNAs that are expressed in benign or malignant thyroid nodules. Furthermore, FNAB-RT-PCR in combination with molecular scanning techniques or direct sequencing provides a valuable screening tool for mutations. The present review highlights the applications of FNAB-RT-PCR method in the identification of new diagnostic molecular markers. The parallel molecular and cytological analysis of FNABs may contribute to a more accurate diagnosis and, thus, to a more specific clinical management of thyroid nodules.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Biopsy, Fine-Needle/*methods,Humans,*Preoperative Care,*Reverse Transcriptase Polymerase Chain Reaction,Sensitivity and Specificity,Sequence Analysis, DNA,Thyroid Nodule/*diagnosis/*genetics/surgery,Thyroidectomy
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/21560
ISSN: 0391-1977
Link: http://www.ncbi.nlm.nih.gov/pubmed/16682941
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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