Glycine maintains mitochondrial activity and bile composition following warm liver ischemia-reperfusion injury (Journal article)

Sheth, H./ Hafez, T./ Glantzounis, G. K./ Seifalian, A. M./ Fuller, B./ Davidson, B. R.

BACKGROUND AND AIM: Experimental studies have shown protective effect by the non-essential amino acid glycine to liver ischemia-reperfusion (I/R) injury but the mechanism of action is unknown. METHODS: A rabbit model of hepatic lobar I/R was used. Three groups of animals (n=6) were studied: Sham group (laparotomy alone), ischemia reperfusion (I/R) group (1 h of liver lobar ischemia and 6 h of reperfusion), and a glycine I/R group (intravenous glycine 5 mg/kg prior to the I/R protocol). Systemic and hepatic hemodynamics, degree of liver injury (bile flow, transaminases), hepatic microcirculation, mitochondrial activity (redox state of cytochrome oxidase), bile composition and cytokines (tumor necrosis factor-alpha and interleukin-8) were measured during the experiment. RESULTS: Glycine administration increased portal blood flow, bile production, hepatic microcirculation and maintained cytochrome oxidase activity as compared with the I/R group during reperfusion. Glycine also reduced bile lactate surge and stimulated acetoacetate release in bile during reperfusion versus the I/R group. Cytokine levels (tumor necrosis factor-alpha, interleukin-8) and hepatocellular injury (aspartate aminotransferase and alanine aminotransferase) were significantly reduced by glycine administration. CONCLUSION: Intravenous glycine administration reduces liver warm I/R injury by reducing the systemic inflammatory response, and maintaining cellular energy production.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Alanine Transaminase/blood,Animals,Aspartate Aminotransferases/blood,Bile/*metabolism,Disease Models, Animal,Electron Transport Complex IV/metabolism,Energy Metabolism/*drug effects,Glycine/administration & dosage/*pharmacology,Hemodynamics/drug effects,Infusions, Intravenous,Interleukin-8/blood,Liver/*blood supply/*drug effects/metabolism,Liver Circulation/drug effects,Microcirculation/drug effects,Mitochondria, Liver/*drug effects/metabolism,Oxidation-Reduction,Rabbits,Reperfusion Injury/metabolism/physiopathology/*prevention & control,Time Factors,Tumor Necrosis Factor-alpha/blood,Warm Ischemia/*adverse effects
ISSN: 1440-1746
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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