Histochemical localization of acid mannose 6-phosphatase activity in the newborn rat hepatocytes (Journal article)

Kalamidas, S. A./ Kotoulas, O. B./ Hann, A. C.


The localization of acid mannose 6-phosphatase activity in newborn rat hepatocytes was demonstrated at the electron microscopic level by using a histochemical method based on the work of Robinson and Karnovsky. Reaction product was virtually restricted to the lysosomes. Most of them exhibited various grades of reactivity. Some were devoid of activity. Our observations suggested that this histochemical method could be used to differentiate distinct subpopulations of lysosomes on the basis of their acid mannose 6-phosphatase activity.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Animals,Animals, Newborn,Hepatocytes/*enzymology,Histocytochemistry,Kinetics,Lysosomes/*enzymology,Phosphoric Monoester Hydrolases/*metabolism,Rats,Rats, Wistar
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/21200
ISSN: 1286-0115
Link: http://www.ncbi.nlm.nih.gov/pubmed/15693419
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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