Hydroxyindole-O-methyltransferase in Y-79 human retinoblastoma cells: effect of cell attachment (Journal article)

Kyritsis, A. P./ Wiechmann, A. F./ Bok, D./ Chader, G. J.


Hydroxyindole-O-methyltransferase (HIOMT), the enzyme in the final step of melatonin synthesis, is present in the Y-79 human retinoblastoma cell line. Using electroblot immunolabellings, a single band corresponding to HIOMT was observed. Immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and quantification of enzyme activity all revealed dramatic increases in HIOMT in cells attached to substrate compared to cells in suspension culture.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Acetylserotonin O-Methyltransferase/metabolism,Animals,Cattle,Cell Line,Cytosol/enzymology,Eye Neoplasms/*enzymology/pathology,Fluorescent Antibody Technique,Humans,Molecular Weight,Retina/enzymology/ultrastructure,Retinoblastoma/*enzymology/pathology,Staining and Labeling
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/21116
ISSN: 0022-3042
Link: http://www.ncbi.nlm.nih.gov/pubmed/2435851
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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