Immunohistochemical analysis of expression of bcl-2 protein in papillary carcinomas and papillary microcarcinomas of the thyroid gland (Journal article)

Mitselou, A./ Peschos, D./ Dallas, P./ Charalabopoulos, K./ Agnantis, N. J./ Vougiouklakis, T.

AIM: To compare the expression of anti-apoptotic protein bcl-2 and tissue proliferation Ki-67 antigen in tissues of human papillary thyroid carcinoma (PTC) and papillary thyroid microcarcinoma (PTMC). METHODS: Bcl-2 protein expression in PTC and PTMC has been evaluated by immunohistochemical analysis. RESULTS: Bcl-2 protein was observed in normal thyroid gland in all the cases; in 25% of the cases of papillary carcinomas the immunoreaction was strong and in 75% of the cases--moderate. In 25% of the cases of papillary microcarcinomas the bcl-2 expression was totally negative and 35% was moderate. Bcl-2 expression was lower in non-sclerosing non-encapsulated (NSNE) (25%) cases of PMCs than in non-sclerosing encapsulated (NSE) (57.15%) cases of PTMCs (p < 0.05). MIB-1/Ki-67 expression was notably reduced or not expressed in normal and benign thyroid tissue. MIB-1 immunoreactivity was present in papillary carcinoma, and on a lower level--in PTMCs. The MIB-1 labelling index of NSE and sclerosing non-encapsulated (SNE) cases was lower than that in NSNE (p < 0.005). CONCLUSION: The present study indicated that NSNE cases of PTMCs may be an early stage in tumor progression, and the bcl-2 and MIB-1 immunohistochemistry may be a valuable tool in predicting the malignant behavior in PMTCs, and contributes to the understanding the growth characteristics of papillary carcinomas of the thyroid gland.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Adult,Aged,Aged, 80 and over,Carcinoma, Papillary/*genetics/pathology,Disease Progression,Female,*Gene Expression Profiling,Humans,Immunohistochemistry,Male,Middle Aged,Neoplasm Staging,Prognosis,Proto-Oncogene Proteins c-bcl-2/*biosynthesis/*genetics,Thyroid Neoplasms/*genetics/pathology
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/21003
ISSN: 1812-9269
Link: http://www.ncbi.nlm.nih.gov/pubmed/15627060
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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