Immunohistochemical study of angiogenesis and proliferative activity in epiretinal membranes (Journal article)

Tsanou, E./ Ioachim, E./ Stefaniotou, M./ Gorezis, S./ Charalabopoulos, K./ Bagli, H./ Peschos, D./ Psilas, K./ Agnantis, N. J.

Formation of epiretinal membranes (ERMs) is a serious complication of retinal diseases, the most important being proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR). In this study, our goal was to (i) calculate the microvessel density (MVD), (ii) evaluate vascular endothelial growth factor (VEGF) expression and (iii) correlate angiogenesis with the proliferative activity as expressed by the expression of Ki67 marker, in both membrane types. We performed immunohistochemistry in 14 PVR and eight PDR membranes, using antibodies against CD34, VEGF, Ki67 and glial fibrillary acidic protein. PDR membranes presented higher average count of microvessels compared with PVR membranes (p = 0.0015). No differences were observed concerning VEGF expression (p = 0.1). The expression of Ki67 was not correlated with microvessel number or VEGF expression. Our study confirms the presence of vascularisation in PDR membranes, as well as the presence of VEGF even in avascular PVR membranes, suggesting that immunoreactivity for VEGF may not be accompanied by angiogenesis.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Cell Proliferation,Diabetic Retinopathy/metabolism/pathology,Epiretinal Membrane/metabolism/*pathology,Humans,Immunoenzyme Techniques,Ki-67 Antigen/metabolism,Retinal Neovascularization/metabolism/*pathology,Vascular Endothelial Growth Factor A/metabolism,Vitreoretinopathy, Proliferative/metabolism/pathology
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/20969
ISSN: 1368-5031
Link: http://www.ncbi.nlm.nih.gov/pubmed/16178982
http://onlinelibrary.wiley.com/store/10.1111/j.1368-5031.2005.00573.x/asset/j.1368-5031.2005.00573.x.pdf?v=1&t=h2lhbhl8&s=5884eac16ebeecb2ca86088b6aecc1e8a5c056ba
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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