Isolation of Escherichia coli O157:H7 from foods in Greece (Journal article)

Dontorou, C./ Papadopoulou, C./ Filioussis, G./ Economou, V./ Apostolou, I./ Zakkas, G./ Salamoura, A./ Kansouzidou, A./ Levidiotou, S.


The presence of Escherichia coli O157:H7 in various foods of animal origin was surveyed in northwestern Greece. Six hundred samples of unpasteurized cows', ewes' and goats' milk, raw minced meat, uncooked frozen beef hamburgers, sandwiches (containing ham or turkey, mixed vegetable salad with mayonnaise and lettuce), fresh traditional Greek pork sausages and swine intestines appropriate for traditional Greek kokoretsi were assayed for E. coli serogroup O157:H7 using the standard cultural method and the immunomagnetic separation technique. The pathogen was detected in 1 out of 100 (1.0%) samples of ewes' milk, 1 out of 75 (1.3%) fresh sausages and 1 out of 50 (2.0%) swine intestines prepared for kokoretsi. The isolated strains were nonsorbitol fermenters, MUG-negative, O157 agglutinating, verotoxin-producing and carried both VT1 and VT2 genes. The three isolated strains were tested for antibiotic resistance and were found to be susceptible to eight antimicrobial agents (ampicillin, chloramphenicol, kanamycin, nalidixic acid, norfloxacin, streptomycin, sulfamethoxazole-trimethoprim and tetracycline).
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Animals,Anti-Bacterial Agents/*pharmacology,Bacterial Toxins/analysis,Cattle,Drug Resistance, Bacterial,Escherichia coli O157/drug effects/*isolation & purification,Food Contamination,*Food Microbiology,Goats,Greece,Humans,Immunomagnetic Separation/methods,Meat Products/*microbiology,Microbial Sensitivity Tests,Milk/*microbiology,Sheep
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/20564
ISSN: 0168-1605
Link: http://www.ncbi.nlm.nih.gov/pubmed/12593930
http://ac.els-cdn.com/S0168160502003136/1-s2.0-S0168160502003136-main.pdf?_tid=21eb2e4c8aae98ffe4e39aa1b025d755&acdnat=1332846077_f0892f2c2e284198705725ce4d221693
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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