Major histocompatibility complex (MHC)-I and MHC-II expression in Hodgkin's disease in relation to the presence of Epstein-Barr Virus (EBV) (Journal article)

Tzardi, M./ Kouvidou, C./ Papakonstantinou, E./ Datseris, G./ Darivianaki, K./ Karidi, E./ Eliopoulos, G./ Delidis, G./ Rontogianni, D./ Kanavaros, P.

We have investigated the immunohistochemical expression of beta2-microglobulin and HLA-DR proteins in Hodgkin's disease (HD) in relation to the expression of the EBV-encoded EBER1-2 mRNAs and the LMP-1 protein. beta2-microglobulin is expressed in association with MHC-I molecules on most nucleated cells and HLA-DR belongs to the MHC-II molecules which are expressed mostly on antigen-presenting cells. Formalin-fixed paraffin embedded tissue from 39 cases of lymphonodal HD were stained by immunohistochemistry for beta2-microglobulin, HLA-DR and LMP-1 proteins and by RNA in situ hybridization for EBER1-2 mRNAs. beta2-microglobulin positive staining was found in Reed-Sternberg and Hodgkin cells (HRS cells) in 18/39 cases of HD. HLA-DR positive staining was found in HRS cells in all cases of HD. EBER1-2 transcripts and LMP-1 protein were detected in HRS cells in 16/39 cases of HD. No correlation as found between the presence of EBER 1-2 transcripts or the LMP-1 protein and the detection of beta2-microglobulin and HLA-DR proteins in HD. Thus, EBV does not seem to use downregulation of MHC-I to avoid the T-cell cytotoxic immune response in HD. In addition, EBV does not seem to be the only factor responsible for the HLA-DR expression in HRS cells of HD, although it could participate in the induction of the expression of HLA-DR molecule in the EBV positive cases of HD.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: *Herpesvirus 4, Human,Histocompatibility Antigens Class I/*analysis,Histocompatibility Antigens Class II/*analysis,Hodgkin Disease/*immunology/*virology,Humans,In Situ Hybridization,RNA, Messenger/analysis,RNA, Viral/analysis,Tumor Virus Infections/*immunology,beta 2-Microglobulin/*analysis
ISSN: 0250-7005
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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