Overexpression of E2F-1 in glioma triggers apoptosis and suppresses tumor growth in vitro and in vivo (Journal article)

Fueyo, J./ Gomez-Manzano, C./ Yung, W. K./ Liu, T. J./ Alemany, R./ McDonnell, T. J./ Shi, X./ Rao, J. S./ Levin, V. A./ Kyritsis, A. P.

The transfer of apoptosis genes to tumors is one of the most promising strategies for cancer gene therapy. We have shown that massive apoptosis occurs when wild-type p53 expression is induced in glioma cells carrying a p53 gene mutation. However, adenovirus-mediated p53 gene transfer is ineffective in causing apoptosis in glioma cells that retain a wild-type p53 genotype. We evaluated the effect of E2F-1 overexpression on the growth of gliomas in vitro and in vivo. In the in vitro study, the adenovirus-mediated transfer of exogenous E2F-1 protein precipitated generalized apoptosis in gliomas. The treatment with Ad5CMV-E2F-1 of nude mice carrying subcutaneous gliomas arrested tumor growth. Our results indicate that E2F-1 has anti-glioma activity in vitro and in vivo.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Adenoviruses, Human/genetics,Animals,Apoptosis/genetics/*physiology,*Carrier Proteins,Cell Cycle Proteins/genetics/physiology,Cell Death/genetics/physiology,Cell Survival/genetics/physiology,Cyclin-Dependent Kinase Inhibitor p16/metabolism,DNA-Binding Proteins/genetics/physiology,Disease Models, Animal,E2F Transcription Factors,E2F1 Transcription Factor,Gene Expression/genetics,Gene Therapy,Genes, Tumor Suppressor,Genetic Vectors/genetics,Glioma/*genetics/physiopathology/therapy,Humans,Mice,Mice, Inbred BALB C,Mice, Nude,Oncogene Protein p21(ras)/metabolism,Recombinant Fusion Proteins/genetics,Retinoblastoma Protein/metabolism,Retinoblastoma-Binding Protein 1,Transcription Factor DP1,Transcription Factors/*genetics/physiology,Transfection/genetics,Tumor Cells, Cultured
URI: https://olympias.lib.uoi.gr/jspui/handle/123456789/19533
ISSN: 1078-8956
Link: http://www.ncbi.nlm.nih.gov/pubmed/9623977
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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