Pre-decondensed sperm head injections into female pronuclei result in chromosomal mingling, zygotic cleavage, and adequate embryonic and fetal development up to delivery of healthy offspring: a novel method of assisted syngamy (Journal article)

Sofikitis, N./ Baltogiannis, D./ Takenaka, M./ Tsoukanelis, K./ Tsambalas, S./ Yamamoto, Y./ Kanakas, N./ Giannakis, D./ Loutradis, D./ Adonakis, G./ Miyagawa, I.


Investigation of the developmental potential post-injection of a pre-decondensed or non-pre-decondensed sperm head into the female pronucleus of a pre-activated oocyte. Rat pre-activated oocytes were treated with intrapronuclear pre-decondensed sperm head injections (IPSHI) (n = 133) or intrapronuclear non-pre-decondensed sperm head injections (INPSHI) (n = 138). All injected oocytes were transferred to pseudopregnant female recipients. Rat IPSHI techniques resulted in the delivery of five healthy offspring. Rat INPSHI techniques did not result in any pregnancies. Rat IPSHI techniques can result in delivery of healthy offspring. Successful performance of human IPSHI techniques might serve as a novel method to manage cases of intracytoplasmic sperm injection failure due to lack of development of male pronucleus or due to failure in pronuclei fusion.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Animals,Cell Nucleus,Chromosomes/*physiology,*Cleavage Stage, Ovum,*Embryonic Development,Female,*Fetal Development,Male,*Microinjections,*Oocytes,Rats,Rats, Wistar,*Reproductive Techniques, Assisted,*Sperm Head
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/19170
ISSN: 0303-4569
Link: http://www.ncbi.nlm.nih.gov/pubmed/15458548
http://onlinelibrary.wiley.com/store/10.1111/j.1439-0272.2004.00622.x/asset/j.1439-0272.2004.00622.x.pdf?v=1&t=h0p8bql0&s=78cb322d65930cc82ab5a5580eeff9b041460083
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

Files in This Item:
File Description SizeFormat 
Sofikitis-2004-Pre-decondensed sper.pdf479.72 kBAdobe PDFView/Open    Request a copy



 Please use this identifier to cite or link to this item:
http://olympias.lib.uoi.gr/jspui/handle/123456789/19170
  This item is a favorite for 0 people.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.