Promoter hypermethylation of the MEG3 (DLK1/MEG3) imprinted gene in multiple myeloma (Journal article)

Benetatos, L./ Dasoula, A./ Hatzimichael, E./ Georgiou, I./ Syrrou, M./ Bourantas, K. L.


BACKGROUND: Methylation represents the most studied epigenetic modification and results in the silencing of genes involved in various processes such as differentiation and cell-cycle regulation. MEG3 represents an imprinted gene maternally expressed in humans that encodes a nontranslated product. In this survey, we studied the methylation status of the specific gene in multiple myeloma (MM). PATIENTS AND METHODS: Twenty-one patients with MM (17 with immunoglobulin [Ig] G, 3 with IgA, and 1 with IgM) were evaluated using methylation-specific polymerase chain reaction (after DNA bisulphite modification). RESULTS: Promoter hypermethylation was observed in 12 (57.14%) bone marrow samples and in 9 of 14 (64.28%) available peripheral blood samples. A correlation with disease stage was also observed and also with the disease subtype (IgG, 64.7%; IgA, 0; IgM, 100%). CONCLUSION: We conclude that promoter hypermethylation of the differentially methylated region of the MEG3 imprinted gene is observed in patients with MM.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Aged,Aged, 80 and over,*DNA Methylation,Female,*Genomic Imprinting,Humans,Immunoglobulins/blood/genetics,Male,Middle Aged,Multiple Myeloma/blood/*genetics,*Promoter Regions, Genetic,Prospective Studies,Proteins/*genetics/metabolism
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/18982
ISSN: 1557-9190
Link: http://www.ncbi.nlm.nih.gov/pubmed/18650181
http://cigjournals.metapress.com/content/l52q2717061r8469/fulltext.pdf
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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