Prothymosin alpha associates with the oncoprotein SET and is involved in chromatin decondensation (Journal article)

Karetsou, Z./ Martic, G./ Tavoulari, S./ Christoforidis, S./ Wilm, M./ Gruss, C./ Papamarcaki, T.

Prothymosin alpha (ProTalpha) is a histone H1-binding protein that interacts with the transcription coactivator CREB-binding protein and potentiates transcription. Based on coimmunoprecipitation and mammalian two-hybrid assays, we show here that ProTalpha forms a complex with the oncoprotein SET. ProTalpha efficiently decondenses human sperm chromatin, while overexpression of GFP-ProTalpha in mammalian cells results in global chromatin decondensation. These results indicate that decondensation of compacted chromatin fibers is an important step in the mechanism of ProTalpha function.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Amino Acid Sequence,Blotting, Western,CREB-Binding Protein,Cell Extracts,Chromatin/*metabolism,Chromosomal Proteins, Non-Histone,Green Fluorescent Proteins/metabolism,HeLa Cells,Histone Chaperones,Humans,Luciferases/metabolism,Male,Mass Spectrometry,Molecular Sequence Data,Molecular Weight,Nuclear Proteins/metabolism,Plasmids/metabolism,Precipitin Tests,Protein Binding,Protein Precursors/genetics/*metabolism,Proteins/chemistry/*metabolism,Recombinant Proteins/metabolism,Sequence Analysis, Protein,Silver Staining,Spermatozoa/metabolism,Thymosin/*analogs & derivatives/genetics/*metabolism,Trans-Activators/metabolism,Transcription Factors,Two-Hybrid System Techniques
ISSN: 0014-5793
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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