Retinoblastoma--origin from a primitive neuroectodermal cell? (Journal article)

Kyritsis, A. P./ Tsokos, M./ Triche, T. J./ Chader, G. J.


The histogenesis of retinoblastoma, the most common intraocular neoplasm of childhood, remains controversial. Previous studies have attributed the origin of the tumour to neuronal, glial or primitive stem cells of retina. In the study described here we have used immunofluorescence to search for the presence of a neuronal marker, neurone-specific enolase (NSE) and a glial marker, glial fibrillary acidic protein (GFAP), in the cells of the human retinoblastoma line Y-79 (ref. 4), before and after successful differentiation into neuronal and glial-like cells. We found that all undifferentiated cells contain both NSE and GFAP, whereas the differentiating neuronal and glial-like cells gradually lose one marker and selectively express the marker that correlates with their morphology. Our results support the notion that retinoblastoma originates from a primitive bipotential (or multipotential) neuroectodermal cell.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Keywords: Cell Differentiation,Cell Line,Ectoderm/cytology,Humans,Intermediate Filament Proteins/metabolism,Neuroglia/pathology,Neurons/pathology,Phosphopyruvate Hydratase/metabolism,Retinoblastoma/*pathology
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/18600
ISSN: 0028-0836
Item type: journalArticle
Link: http://www.ncbi.nlm.nih.gov/pubmed/6694739
Item language: en
Item access scheme: campus
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Επιστημών Υγείας. Τμήμα Ιατρικής
Publication date: 1984
Abstract: The histogenesis of retinoblastoma, the most common intraocular neoplasm of childhood, remains controversial. Previous studies have attributed the origin of the tumour to neuronal, glial or primitive stem cells of retina. In the study described here we have used immunofluorescence to search for the presence of a neuronal marker, neurone-specific enolase (NSE) and a glial marker, glial fibrillary acidic protein (GFAP), in the cells of the human retinoblastoma line Y-79 (ref. 4), before and after successful differentiation into neuronal and glial-like cells. We found that all undifferentiated cells contain both NSE and GFAP, whereas the differentiating neuronal and glial-like cells gradually lose one marker and selectively express the marker that correlates with their morphology. Our results support the notion that retinoblastoma originates from a primitive bipotential (or multipotential) neuroectodermal cell.
Journal name: Nature
Journal type: peer-reviewed
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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