Effect of chromatographic parameters and detector settings on the response of HILIC-evaporative light-scattering detection system using experimental design approach and multicriteria optimization methodology (Journal article)

Karatapanis, A. E./ Fiamegos, Y. C./ Sakkas, V. A./ Stalikas, C. D.


Four polar compounds, i.e. pantothenic acid, inositol, taurine and caffeine were used as probe solutes in conjunction with chemometric methods to find out meaningful implications of chromatographic conditions and detector settings on the system performance. Putting a premium on the conditions of hydrophilic interaction liquid chromatography (HILIC) and settings of evaporative light-scattering detection (ELSD), we scrutinize the importance of certain factors on signal-to-noise ratio and its variability. The application of a central composite design reveals that caffeine, which sublimes, differentiates from the relatively thermosensitive pantothenic acid as well as from inositol and taurine, which are thermostable, do not sublime and have high melting points. It seems that prior knowledge of solute characteristics is critical to estimate the chromatographic response as a function of chromatographic conditions and detection settings. Reducing the responses to just one by combining them "ad hoc", results in an overall desirability function, which brings out the global optimal chromatographic conditions and detector settings. (c) 2010 Elsevier B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: experimental design,desirability functions,elsd,hilic,hydrophilic-interaction chromatography,liquid-chromatography,polar compounds,separation,validation,acid
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/10470
ISSN: 0039-9140
Link: <Go to ISI>://000286718700009
http://ac.els-cdn.com/S0039914010004960/1-s2.0-S0039914010004960-main.pdf?_tid=119d834c29f9b5c76127210c31e7f9be&acdnat=1333039255_27146eb81110852991160c32c16b614c
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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