Determination of plasma, urine, and bovine serum albumin low-molecular-weight carbonyl levels by capillary gas chromatography with electron-capture and mass-selective detection (Journal article)

Konidari, C. N./ Giannopoulos, T. S./ Nanos, C. G./ Stalikas, C. D.


Peroxidation of lipids produces low-molecular-weight carbonyl compounds, which are reactive with biological nucleophiles. The analysis of these compounds is often difficult. A multicomponent method for the determination of 11 of them in biological samples is reported. The samples are subjected to a pretreatment-derivatization procedure followed by gas chromatographic analysis with either electron-capture detection (ECD) or mass-selective detection (MSD) in the selected-ion monitoring mode. The procedure involves derivatization of the analyte with 2,4,6-trichlorophenylhydrazine, extraction with n-hexane, and separation of the derivatization products on a nonpolar gas chromatographic column. The concentration of the derivatization reagent, pH, reaction time, temperature, and presence of extraneous ions were investigated to determine the optimal derivatization conditions. Under these conditions, the method allows for the selective detection of low-molecular-weight carbonyl compounds at femtomole levels in several biological materials such as plasma, urine, and bovine serum albumin without interferences. The limits of detection were in the ranges 0.01-0.2 muM for ECD and 0.15-1.5 muM for MSD. The mean procedural recoveries obtained during the method validation were within the range 85-95% and the intra- and interassay standard deviations do not exceed 4.6 and 6.1%, respectively. (C) 2004 Elsevier Inc. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: low-molecular-weight carbonyl compounds,biological samples,2,4,6-trichlorophenylhydrazine,gas chromatography,low-density lipoproteins,performance liquid-chromatography,lipid-peroxidation products,hydroxy fatty-acids,biological samples,free malonaldehyde,protein oxidation,linoleic-acid,malondialdehyde,spectrometry
URI: http://olympias.lib.uoi.gr/jspui/handle/123456789/10350
ISSN: 0003-2697
Link: <Go to ISI>://000227223000008
http://ac.els-cdn.com/S0003269704008851/1-s2.0-S0003269704008851-main.pdf?_tid=f588ad87d28680455d2bee954f42f466&acdnat=1333040851_d403180aae8d9b34e7900f1472c8012e
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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