Determination of okadaic acid, dinophysistoxin-1 and related esters in Greek mussels using HPLC with fluorometric detection, LC-MS/MS and mouse bioassay (Journal article)

Louppis, A. P./ Badeka, A. V./ Katikou, P./ Paleologos, E. K./ Kontominas, M. G.

An approach involving both chemical and biological methods was undertaken for the detection and quantification of the marine toxins okadaic acid (OA), dinophysistoxin-1 (DTX-1) and their respective esters in mussels from different sampling sites in Greece during the period 2006-2007. Samples were analyzed by means of a) high performance liquid chromatography with fluorometric detection (HPLC-FLD), using 9-athryldiazomethane (ADAM), as a pre-column derivatization reagent, b) liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and c) the mouse bioassay. Free OA and DTX-1 were determined by both HPLC-FLD and LC-MS/MS, while their respective esters were determined only by LC-MS/MS after alkaline hydrolysis of the samples. The detection limit (L.O.D.) and quantification limit (L.O.Q) of the HPLC-FLD method were 0.015 mu g/g HP and 0.050 mu g/g HP, respectively, for OA. The detection limit (L.O.D.) and quantification limit (L.O.Q.) of the LC-MS/MS method were 0.045 mu g/g HP and 0.135 mu g/g HP, respectively, for OA. Comparison of results between the two analytical methods showed excellent agreement (100%), while both HPLC-FLD and LC-MS/MS methods showed an agreement of 97.1% compared to the mouse bioassay. (C) 2009 Elsevier Ltd. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: diarrhetic shellfish poisoning,okadaic acid,dinophysistoxins,esters of okadaic acid,mussels,mouse bioassay,hplc,lc-ms/ms,chromatography-mass-spectrometry,diarrhetic shellfish toxins,performance liquid-chromatography,lipophilic toxins,marine toxins,portuguese bivalves,poisoning toxins,thermaikos gulf,fatty-acids,dsp toxins
ISSN: 0041-0101
Link: <Go to ISI>://000275705300006
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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