Determination of hydrogen peroxide by using a flow injection system with immobilized peroxidase and long pathlength capillary spectrophotometry (Journal article)

Pappas, A. C./ Stalikas, C. D./ Fiamegos, Y. C./ Karayannis, M. I.

The development of a highly sensitive method for the determination of nanomolar concentrations of hydrogen peroxide in the liquid phase is described. This paper demonstrates for the first time a flow injection analysis (FIA) system with immobilized enzyme reactor combined with a total internal reflective cell (a liquid waveguide capillary cell (LWCC)) and spectrophotometric detection, for the development of an improved procedure for the determination of hydrogen peroxide. Moreover, the newly synthesized 4-aminopyrazolone derivative, 4-amino-5-(p-aminophenyl)-1-methyl-2-phenyl-pyrazol-3-one (DAP), is used as a color coupler in its oxidative condensation with the sodium salt of N-ethyl-N-sulphopropylaniline sodium salt (ALPS) which acts as a hydrogen donor. Immobilization of peroxidase is achieved by coupling the periodate-treated enzyme to aminopropyl controlled-pore glass (CPG) beads. The determination of hydrogen peroxide is carried out in a 0.1 M phosphate buffer and the product is monitored at 590 nm with a charge-coupled device (CCD) detector equipped with fiber optics in a fully computerized system. The interference of different species, mainly ionic, was investigated. The method permits detection down to 4 nmol l(-1) hydrogen peroxide (signal-to-noise ratio = 3). A linear calibration graph was obtained over the range 20-700 nmol l(-1). The relative standard deviation (R.S.D.) at 300 nmol l(-1) H,02 is 1.7% (n = 7), The method was successfully applied for the determination of hydrogen peroxide in samples from a vat-cleaning process. (C) 2002 Elsevier Science B.V. All rights reserved.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: hydrogen peroxide,liquid waveguide capillary cell,immobilized peroxidase,flow injection analysis,amperometric determination,fluorometric-determination,horseradish-peroxidase,biosensor,water,chemiluminescence,electrode,reagent,samples,column
ISSN: 0003-2670
Link: <Go to ISI>://000174586200015
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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