Complementary segmental labeling of large RNAs: economic preparation and simplified NMR spectra for measurement of more RDCs (Journal article)

Tzakos, A. G./ Easton, L. E./ Lukavsky, P. J.

NMR structure determination of large RNAs is often restricted by limited RDC information caused by chemical shift degeneracy. We established a general, time- and cost-effective methodology for the preparation of 13C/15N complementary labeled RNAs from a single plasmid. Applying this method to the 25 kDa BC1-DTE RNA, we were able to resolve severe chemical shift degeneracy, thereby almost doubling the number of RDC restraints in comparison to the conventional 13C,15N uniform-labeled RNA.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: Carbon Isotopes,Imines/chemistry,Isotope Labeling/*methods,Magnetic Resonance Spectroscopy/methods,Nitrogen Isotopes,Nucleic Acid Conformation,Pharmaceutical Preparations/*chemical synthesis/*economics,RNA/*chemistry,Transcription, Genetic
ISSN: 0002-7863
Publisher: American Chemical Society
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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