Changes in the Cd Spectra of Calf Thymus DNA Induced by Sequential Polypeptides in Aqueous-Solutions .1. (Journal article)

Tsikaris, V./ Panoupomonis, E./ Sakarellos, C./ Sakarellos-Daitsiotis, M./ Marraud, M.

Interactions of calf thymus DNA with sequential polypeptides were studied using c.d. spectroscopy in aqueous solutions. It was found that DNA structural alterations induced by sequential polypeptides (L-Arg-X-Gly)n (where X = L-Val, Leu, Ile, Nva, Nle) are modulated by the nature of the X residue. Thus, the polypeptide (L-Arg-L-Nva-Gly)n induced the 10.2B-DNA form, whereas the polypeptides (L-Arg-L-Ile-Gly)n having one methyl group less on the X residue side chain, did not provide any significant modification to the structure of DNA. The effect of ionic strength from 0.14 M NaCl (physiological value) to zero was also analysed on the basis of the observed c.d. changes and the degree of complexation in the DNA-polypeptides was estimated.
Institution and School/Department of submitter: Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Χημείας
Keywords: sequential polypeptides,calf thymus DNA,cd spectroscopy,circular-dichroism,histone models,basic polypeptides,nucleic-acids,deoxyribonucleic acid,thermal-denaturation,chromatin models,conformation,arginine,complexes
ISSN: 0141-8130
Link: <Go to ISI>://A1991GW72800006
Publisher: Elsevier
Appears in Collections:Άρθρα σε επιστημονικά περιοδικά ( Ανοικτά)

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